![]() ![]() It was inferred that the significant expression of this gene may cause cytoplasmic male sterility in soybeans. This fragment had a high expression level in the soybean flower buds of the cytoplasmic male sterile line and a lower expression level in the soybean flower buds of the maintainer line. A homologous MADS gene fragment has been cloned from the flower buds of NJCMS2A. MADS-box genes play an essential role in flower development and flower organ formation. In Arabidopsis, the overexpression of soybean GmFULc leads to early flowering, and the relative expression of flowering-related genes FT, SOC1, and LFY increases, which plays a role in promoting flowering. The expression of the FT gene is inhibited by the MADS-box family FLC homolog, GmFLC-like, resulting in delayed flowering. MADS-box genes regulate plant flowering time by responding to the external light environment and regulating endogenous signal changes. The MADS-box genes affect flowering time, flower development, seed development, and other processes, and have been shown to participate in the regulation of soybean ( Glycine max (Linn.) Merr.) growth and development. Class E genes include SEP, which can be further divided into four types, namely SEP1, SEP2, SEP3, and SEP4, which are involved in regulating Arabidopsis flower development. AG, SHP1, AGL1, SHP2, and STK belong to C/D class genes. ap3 and pi mutants have identical phenotypes by producing the sepal structure and the carpel in Arabidopsis ( Arabidopsis thaliana). PI and AP3 belong to class B genes in the MADS family. AP1 is classified as a class A gene, and is a meristem and floral organ recognition gene that promotes the development of petals and sepals. The formation of sepals, petals, stamens, carpels, and ovules is influenced by the interaction of these five types of genes. Most MADS genes that have been reported are type II genes, such as floral homologs from the ABCDE model that belong to the MIKCC subfamily. Type I genes are divided into Mα, Mβ, and Mγ types, according to different gene structures, while type II genes are divided into MIKCC and MIKC* types. Type I MADS-box genes contain the SRF domain, and type II MADS-box genes contain the K region, which is less conserved. ![]() The MADS-box gene family is divided into type I and type II according to their different domains. The results of this study lay the foundation for further research on the biological functions of MADS transcriptional factors in soybeans. In conclusion, GmAP3 may directly or indirectly affect the flower development of soybean. The average flowering time of overexpressed soybean and tobacco plants was 6–8 days earlier than that of wild-type plants, and the average flowering time of gene-edited soybean and tobacco plants was 6–11 days later than that of wild-type plants. A follow-up analysis showed that overexpression of the GmAP3 gene advanced flowering time and resulted in changes in floral organ morphology. Yeast two-hybrid assays demonstrated that GmAP3 interacts with AP1 to determine the identity of flower organ development. The expression of GmAP3 is closely related to the expression of essential enzyme genes related to flower development. Here, the full-length cDNA sequence of GmAP3 was obtained by RACE and it was verified that it belongs to the MADS-box subfamily by a bioinformatics analysis. However, the function of the soybean AP3 genes in flower development is unknown. AP3 has been studied and is reported to affect structural changes in floral organs in various plants. ![]()
0 Comments
Leave a Reply. |